In Vivo study of PCL/Lignin Nanocomposite Conduit for Promoting Sciatic Nerve Regeneration in a Rat Sciatic Nerve across a Gap

Background:Injuries of the peripheral nervous system (PNS) lead to permanent functional loss of target tissue and neuropath-ic pain and reduce life quality. The regeneration of damaged peripheral nerve has become a difficult problem. For periph-eral nerve regeneration, nerve autograft has been considered as the gold standard for the restoration of nerve regeneration. But autograft is limited by the short-term accessibility of donor nerves, increasing illness, and scar formation at the donor site. Material and Methods: Following the production of PCL/ Lignin conduit by Electrospinning technique and Surgical im-plantation conduit characterized by, Scanning Electron Micro-scope (SEM) and Transmission Electron Microscope (TEM), electrophysiological assessment, hematoxylin-eosin (HE), Terry Chromosome and toluidine blue staining, Immunohisto-chemistry (IHC).Results: This investigates present that PCL/Lignin conduit can have the good accelerating potential for neural length and pro-moting regeneration sciatic nerve.Conclusion: Our results propose that PCL/Lignin serve as a new biodegradable artificial nerve guide for nerve tissue engi-neering.

Keywords:

Peripheral Nerve Injuries , Biodegradable Nerve Con-duit , Electrospinning , Sciatic Nerve Regeneration