Cancer Stem Cell Enrichment and Neurosphere Formation in Isolated Cells from Glioblastoma Tumor Sample

Background: Glioblastoma multiform (GBM) is the most common malignancy in the central nervous system account-ing for 45% of the malignant brain tumor and the most lethal type of brain cancer. The major problem on the way of effective treatment for this type of cancer is presence of group of cells that called cancer stem cell in tumor mass. Research has shown that cancer stem cell paly role in metastasis, tumor resistance to chemotherapy and radiotherapy and tumor recurrence after treatment.Materials and Methods: At first glioblastoma cells were iso-lated from tumor tissue then a group of cells were cultured in NMGF (Neurosphere medium supplemented with Growth Fac-tors) and another one were examined for cancer stem cell popu-lation (CD133+) by flow cytometry. After 25 days, a group of cells were checked for CD133+ by flowcytomery.Results: After 7 days first neurosphere formed and Result of CD133+ flow cytometry in zero day was 1.58% and after 25 days was 5.64%.Conclusion: Neurosphere formation shows increasing of can-cer stem cell population and Result of CD133+ flow cytometry indicate the cancer stem cell present that was 1.58% in zero day, was increased to 5.64% in 25 day. Sphere formation in NMGF was supported by result of CD133 flow cytometry in 25th day.

Keywords:

Glioblastoma , Brain Cancer , Cancer Stem Cell