Background: Acute myeloid leukemia is a malignant disor-der characterized by abnormal growth and differentiation of primary hematopoietic stem cell and leads to accumulation of immature myeloid precursors in the blood and bone marrow. The spread of immature myeloid cells is accompanied by the normal production of other differentiated cells, including red blood cells and platelets. In many studies,
miR-625 has been shown to inhibit the downstream pathways involved in the in-vasion and metastasis of the integrin –linked kinase(ILK) sign-aling pathway, and also it has been proved that expression of
miR-625 has decreased in acute myeloid leukemia cell lines. Therefore, the increase expression of
miR-625 may be caused by induction of apoptosis, reduction of metastasis and invasion in cancer cells of acute myeloid leukemia by reducing the as-sociated oncogenes. The aim of this study was to investigate the effect of
miR-625 on invasion via the
ILK pathway in KG-1 cell line. In order to investigate the mechanism of
miR-625 ef-fect on KG1 cell invasion, the important oncogenes of the
ILK pathway were investigated.Materials and Methods: The KG1 cell line was transfected with a vector containing pLenti-miR-625-5p-GFP and pLenti-Backbone-GFP by viral method. Then, confirming the increase of
miR-625 expression by q-RT-PCR, the amount of cell inva-sion in the laboratory environment was assessed by invasion as-say. Finally, in order to investigate the mechanism of
miR-625 effect, the levels of COX2, NF-ÁB, GSK3, MMP9, AP1, AKT,
ILK at the gen level by q-RT-PCR and on the protein level were evaluated proteins NF -κB and MMP-9 were investigated by western blotting. Also, CXCR4 levels were assessed by flow cytometric as invasive and homing cell markers.Results: Investigation of invasive KG1 cells transfected by miR-625-5p structure showed a significant decrease in the invasion.
ILK gene expression in these cells significantly de-crease compared to the control group (Backbone). The expression of NF-κB, COX2 showed a significant decrease compared to the control group, and expression of MMP9, AP1 and AKT increased significantly, whereas GSK3β did not change signifi-cant. Changes in the expression of MMP9 and NF-κB protein were observed so that level protein of NF-κB is decreased and MMP9 don’t have significant change. To evaluate the expres-sion of CXCR-4 by flow cytometry, the expression of CXCR4 in the
miR-625 group was significantly lower than that of the control group.Conclusion: Increasing the expression of miR-625-5p leads to decrease cellular invasion in AML cells and can be considered as a new strategy to help treat AML patients in the future. How-ever, further studies are needed to achieve this goal.