Human Embryonic Stem Cells Derived Mesenchy-mal Stem Cells Conditioned Medium for Chondogenic In-duction of Human Embryonic Stem Cells

Background: Osteoarthritis (OA) is one of the most com-mon joint diseases in the world. Cell therapy considered as a novel approach for OA treatment. Human embryonic stem cells (hESCs) because of their unlimited self-renewal and innate pluripotency, can be useful source for human articular cartilage regeneration and future OA cell-based therapies. Recently, it was shown that conditioned medium (CM) from meneschymal stem/stromal cells (MSCs) can induce chondrogenic differen-tiation of hESCs. Here, the chondcogenic induction of hESCs-derived mesenchymal stem/stromal cells CM (hESCs-MSCs CM) was investigated in a 3D culture for 4 days.Materials and Methods: CM from Yazd2 (hESCs; 46,XY)derived MSCs was collected and stored at -20ºC. Embryoid body (EB) formation from Yazd4 (hESC line; 46,XX) was done as 3D culture in spontaneously differentiation (SD) and conditioned medium (CM) groups for 4 days. Subsequently, EBs from both groups were collected and RNA extraction, C-DNA synthesis and RT-PCR was performed for the β-ACTIN, MEOX1, COL2A1, RUNX2 genes.Results: Our data indicated that after 4 days, the EBs in CM group expressed all the genes which were studied for chondro-genesis of hESCs, although, the EBs in SD group expressed none of them.Conclusion: Our data shows that hESC-MSC CM induces chondrogenic differentiation to hESCs as shown by the gene expression data using RT-PCR. This finding opens new insights for better understanding of cellular, molecular, developmental and environmental factors in human chondrogenesis for further studies in developmental biology, toxicology and drug discov-ery to cure OA.

Keywords:

Chondrogenesis , Conditioned Medium , Embryoid Body , Human Embryonic Stem Cells , Mesenchymal Stem/ Stromal Cells