Microfluidic Approaches for Separation of Mouse Tumoral Cells from Neonate Spermatogonial Cells Suspen-sions

Background: Some male survivors of childhood cancer are azoospermic. spermatogonial stem cells(SSC) isolation and pu-rification are really important.The aim of this study was sepa-rating the mouse malignant cell line (EL-4) and SSC in vitro. Materials and Methods: SSC were isolated 60 neonatal mice were used and co-cultured with EL-4 and divided into 2 groups: 1) Control (co-culture in culture medium), 2(Co-cultured cells were separated by microfluidic device. The percent of cells were assessed by flow cytometry and immunocytochemistry and confirmed by RT-PCR.Results: The percentage of EL-4 and SSC after isolation was collected at two outlets, the output for smaller outlet was for SSC (0.12%) and EL-4 (42.14 %) While in larger outlet was SSC (80.38 %), EL-4 (0.32%) in control group, the percentage of cells were SSC (21.44%), EL-4 (23.28 %).Conclusion: The present study demonstrates the use of elec-trophoretic Microfluidic device is effective in separating cancer cells from spermatogonial stem cells.

Keywords:

Microfluidic Device , Spermatogonial Stem Cell , Mouse Cancerous Cell , Cell Sorting