Human SRY May Play A Negative Role in Conver-sion of Primed to Naïve Human Pluripotent Cells

Background: Both of primed and naïve state are pluripotent stem cells but exhibit slightly different properties. The identifi-cation of signaling pathways which differences between these two cells can be useful to the conversion of primed to naïve state.Materials and Methods: In this study, we analyzed the expres-sion data of the UGent11.7 cell line (XY cell line) which previ-ously reported. By the loss of function approach, we investi-gated the role of Y chromosome genes under the primed state using specific siRNA treatment. The findings were validated by gene and protein expression by (qRT-PCR) and western blot respectively.Results: We found that most of the Y chromosome genes like SRY showed high expression level in primed versus naïve hESCs. Therefore, we hypothesize that SRY prevents WNT/β-catenin signaling by interaction and inhibition of β-catenin translocation. By the loss of function approach through deple-tion of SRY on the primed cells, we observed that the expres-sion of pluripotency markers and WNT signaling target genes such as Brachuary, GCBM, TBX2, and TBX3 increased in si-SRY treated cells.Conclusion: In conclusion, we revealed that inhibition of SRY results in nucleus translocation of β-catenin and up-regulation of WNT signaling pathway which important to naïve conver-sion.

Keywords:

Human Embryonic Stem Cell , Naïve , Primed , SRY , Wnt Signaling Pathway