Bone Marrow Mesenchymal Stem Cells and L-Car-nitine Co-Treatment Suppress Oxidative Stress and Inflam-mation in Mice with Induced Polycystic Ovary Syndrom

Background: Polycystic ovary syndrome (PCOS) is a com-mon cause of infertility due to anovulation. Bone marrow mesenchymal stem cells (BM-MSCs) have anti-inflammatory and anti-oxidant properties which may inhibit oxidative s tress and inflammation and L-Carnitine can improve the survival rate of transplanted MSCs. Since inflammation and oxidative stress are involved in the pathogenesis of PCOS, we decided to investigate the effect of BM-MSCs and L-Carnitine co-treatment on oxidative Stress and inflammation in mice with induced PCOS.Materials and Methods: PCOS was induced through daily in-jections of testosterone enanthate (1 mg/100g S.C. for 5 weeks). NMRI mice (3 weeks old) were divided into 5 groups (n=6): Control, PCOS, PCOS + L-Carnitine, PCOS + BM-MSCs and PCOS + L-carnitine + BM-MSCs. Treatment of L-Carnitine was carried out with the dose of 500 mg/kg by i.p injections every other day for 4 weeks. BMMSCs were injected through the tail vein (106MSCs/30g body weight) at 1 and 14 days after induction of PCOS. At the end of treatment, serum levels of IL-6 and TNF-α, Malondialdehyde (MDA) and the antioxidant capacity were measured relatively using the ELISA kit, thio-barbituric acid (TBA) and Ferric reducing antioxidant power (FRAP) assay. Data were analyzed using one way ANOVA and Tuckey s test and the means were considered significantly dif-ferent at P<0.05.Results: The Serum levels of MDA, IL-6 and TNF-α signifi-cantly increased in the PCOS groups compared to the control group. These parameters significantly reduced in treated PCOS groups. IL-6 and TNF-α only in the PCOS + L-carnitine + BM-MSCs group were compensated to control level. Antioxidant capacity also reduced significantly in the PCOS group when compared to the control while it significantly increased in the treated PCOS groups to the control level.Conclusion: We conclude that BM-MSCs and L-carnitine have protective effects against inflammation and oxidative stress in mice with induced PCOS.

Keywords:

Polycystic Ovary Syndrome , BM-Mscs , Stress Oxi-dative , Inflammation , Mouse