The Evaluation of Diazinon Effects on Sperm Matura-tion and Sperm DNA Denaturation in Infertile Men

Background: Exposure to pesticide is one of the important reason for infertility. The aim of this study investigation of diazinon effects on the sperm maturation and sperm DNA dena-turation in infertile men in Babol cityMaterials and Methods: 90 adult males (18-52 years old) were divided in to three groups (n=30). Group 1(infertile and Diazinon-exposed men), Group 2(infertile and Diazinon-unex-posed men) and Group 3(fertile and Diazinon-unexposed men) as a control. Sperm maturation and sperm DNA denaturation were evaluated by aniline blue staining and acridin orange test Respectively . Collection Data were analyzed by spss software (One way ANOVA test). Signification different at P<0.05.Results: The percentage of immature sperms increased signifi-cantly in diazinon-exposed infertile group and the diazinon-un-exposed infertility group compared to control group (p <0.000). Also, percentage of this parameter was higher in diazinon-exposed infertile group than diazinon-unexposed infertility group, but was not significant. The percentage of sperm DNA denaturation increased significantly in diazinon-exposed infer-tile group (P <0.000) and diazinon-unexposed infertile group (P <0.002) compared to control group, and the percentage of mentioned parameter was higher in the diazinon-exposed infer tility group than diazinon-unexposed infertility group, but was not significant.Conclusion: The results of this study show that Diazinon sig-nificant effect on the sperm maturation, sperm DNA denatura-tion and fertility in men. Consequently it is necessary to restrict the use of Diazinon in a systematic program.

Keywords:

Diazinon , Sperm Maturation , Sperm DNA Denatura-tion