Positive Effects of Bioactive Peptides on Sperm Viability and Motility in Male Rate Treated with Cimetidine

Background: Cimetidine is widely used for digestive tract ul-cers but induces testis injury. Bioactive peptides are extracted from protein sources and a number of studies have suggested as a beneficial agent during peroxidative damage. In this study positive effects of Bioactive peptides on sperm viability and motility in male rate treated with cimetidine was investigated. Materials and Methods: Forty- two male rats (165±20 g) were randomly divided into six groups of seven animals and were fed with treatments (Control, peptides 20 mg/kg, cimetidine 40mg/ kg, cimetidine 120mg/kg, peptides+ 40 mg/kg cimetidine, pep-tides + 120 mg/kg cimetidine) for 6 weeks. At the end of the experiment, all the animals were anesthetized with chloroform, the vase deferans separated. The eosin-negrosine dyeing and Neubauer lams were used to determine sperm viability and mo-tility respectively.Results: The results showed that dietary treatments signifi-cantly affected on viability of sperm and the highest value was observed in the animals fed bioactive peptides treatments which had significant difference with cimetidine treated rats (P<0.05). No significant difference was seen in sperm motility in animals (P> 0.05).Conclusion: Based on our results, boactive peptides extracted from marine sources improve viability of sperm in cimetidine treated rats but did not affect the sperm motility. Aforementioned results can be explained according to bioactive peptides antioxidant activity which reported in several references.

Keywords:

Cimetidine , Bioactive Peptides , Reproduction