Effects of Myo-inositol on Sperm Parameters and DNA Integrity in Asthenozospermia after Cryopreservation
Publish place: 20th congress on reproductive biomedicine and 15th congress on stem cell biology & technology
Publish Year: 1398
نوع سند: مقاله کنفرانسی
زبان: English
View: 443
نسخه کامل این Paper ارائه نشده است و در دسترس نمی باشد
- Certificate
- من نویسنده این مقاله هستم
این Paper در بخشهای موضوعی زیر دسته بندی شده است:
استخراج به نرم افزارهای پژوهشی:
شناسه ملی سند علمی:
RROYAN20_284
تاریخ نمایه سازی: 29 مهر 1398
Abstract:
Background: Human semen cryopreservation considered as indispensable part of assisted reproductive centers, andrology laboratories and sperm bank. Despite long history of semen cryopreservation, post thawing survival rate is still limited and fails to meet the ideal expectation. Accordingly, cryoprotective media are commonly supplemented with antioxidants and it has been shown to improve cryo-survival post thawing. Therefore, the aim of this of this study was to evaluate effect of myo-ino-sitol during human semen cryopreservation.Materials and Methods: A total of 20 semen samples were collected from men with asthenozospermia parameters attended the Andrology Unit of the Qom Fertility and Infertility Center. Each semen sample was divided into two equal aliquots. The two identical aliquots of each semen sample were randomized into two groups (A and B). Group A was treated with cryo-pro-tectant plus with (2 mg/mL) Myo-inositol solution, while group B was treated with cryo-protectant alone (control). The Sperm parameters after thawed analysis by WHO guidelines and DNA integrity were evaluated via acridin orange method.Results: The results of this study showed that the sperm sam-ples frozen with cryoprotectant (Myo-inositol) had a signifi-cantly higher proportion of sperm motility and sperm viability compared with those frozen without cryoprotectants (P<0.01). In addition, this study showed DNA integrity had significant difference between groups (P<0.05).Conclusion: In vitro Myo-inositol supplementation of cryo-preserved ejaculate sperm, from infertile men, resulted in a signifiicant enhancement of post-thaw sperm quality. Such fiinding is interesting, and may have important implications on the future outcome of assisted reproductive techniques using cryopreserved sperm.
Keywords:
Authors
M Azizi
Department of Biology, Faculty of Science, Arak University, Arak,Iran
M Soleimani Mehranjani
Department of Biology, Faculty of Science, Arak University, Arak,Iran
E Cheraghi
Department of Biology, Faculty of Science, Arak University, Arak,Iran