The Effects of Myo-Inositol on Mitochondrial Func-tion and Oxidative Stress Condition in MII Oocytes of PCOS Model Mice

Background: Polycystic ovary syndrome (PCOS) is associ-ated with hyperandrogenism, polycystic ovaries, infertility due to ovarian dysfunction and menstrual irregularity and ultimately oocytes whit poor quality. The administration of myo -inositol (MYO)was associated with a decreased of se-rum testosterone, increase insulin sensitivity and improves the oocytes quality. Inofolic is a dietary supplement for women with PCOS that contains myo-inositol(2 g) and folic acid(200 mcg).Materials and Methods: Female NMRI mice were treated with a vehicle control or DHEA (6 mg /100 g body weight) or DHEA plus inofolic (0.37 mg /g body weight) for 20 con-secutive days. After 20 days, Mature oocysts (MII) were re-trieved from isolated ovaries . For inner mitochondrial mem-brane potential (MMP) or reactive oxygen species (ROS) or reduced glutathione (GSH) staining, denuded MII oocytes were incubated in PBS–PVA containing JC-1, H2DCFDA and Cell Tracker Blue fluorochromes respectively. After incubation, oo-cytes were washed and fluorescence was observed using a fluo-rescence microscope and fluorescence intensity was measured with Image J software.Results: Significantly GSH and MMP were lower and ROS was higher in DHEA-treated oocytes compared with vehicle-treated. In DHEA+inofolic group, GLUT and MMP were sig-nificantly increased in contrast to the DHEA-treated and, On the contrary ROS fell.Conclusion: It is likely that myo-inositol plays an important role in reducing ROS, increasing the antioxidant capacity, re-ducing cellular apoptosis and follow by increasing MMP and ultimately improving mitochondrial function in mice MII oo-cytes of PCOS model.

Keywords:

Myoinositol , Polycistic Ovary Syndrome , Reactive Oxygen Species , Reduced Glutathione , Inner Mitochondrial Membrane Potential