Effect of the Time Interval between The Injection of HCG and The Time of ICSI on Assisted Reproduction Out-comes

Background: Assisted Reproductive Technology (ART) re-sults depend on many factors which affect follicular develop-ment, oocyte maturation, fertilization and subsequently embryo development. One of the factors is the time interval between the injection of hCG and the time of Intracytoplasmic Sperm Injection (ICSI) .The aim of this study was to effect of the time interval between the injection of hCG and the time of ICSI on assisted reproduction outcomes.Materials and Methods: A total of 102 women referred in-fertility treatment center of besat hospital, sanandaj underwent IVF/ICSI cycles during the study period. The metaphase II oo-cytes were retrieved after 36 hours of hCG injection. The cycles were divided into 2 groups: in group I, ICSI was done after hours38-40 from the hCG injection (including 2-4 hours of oo-cyte incubation). In group II, ICSI was performed after 42—44 hours from the hCG injection (including 6-8 hours of oocyte incubation).Results: A total of 765 metaphaseIIoocytes were obtained of which ICSI was performed in 366 oocytes in group I and 380 in group II (P > 0.05). The remaining oocytes were conventionally inseminated (IVF). There was a significant difference between the two groups in the fertilization, pregnancy, and implantation rate, a higher pregnancy and implantation rate was found in group II (P<0.05).Conclusion: The present study indicated the time interval be-tween the injection of hCG and the time of ICSI affected on assisted reproduction outcomes.

Keywords:

ART , IVF , ICSI , hCG ,