Background: Despite several studies in general population, there is no direct evidence regarding the cut-off levels of pre-pregnancy body mass index (BMI) and first trimester fasting glucose (FG) to predict the risk of developing GDM in infer-tile women as a high risk population. Accordingly, the present study was designed to evaluate the predictive value of BMI and FG to predict GDM risk, and then to determine the cut-points of BMI, FBS and the combination of two biomarkers (BMI+ FG) for diagnosis of at risk pregnant women conceived by ART to target clinical surveillance in a more effective manner.Materials and Methods: In this case-control study, 270 sin-gleton pregnant women consisted of 135 (GDM) and 135 (non-GDM) who conceived using ART were assessed. The diagnosis of GDM was confirmed by a one-step glucose tolerance test (O-GTT) using 75 g oral glucose. BMI was classified base on World Health Organization (WHO) criteria. The association be-tween BMI, FG, and BMI+FG with the risk of GDM develop-ment was determined by logistic regression and adjusted for confounding factors. Receiver operating characteristic (ROC) curve analysis was performed to evaluate the value of BMI, FG, and BMI+FG for the prediction of GDM.Results: There were significant differences between GDM and non-GDM groups in terms of maternal age, BMI, fam - ily history of diabetes, and history of polycystic ovary syndrome (P<0.05). The overweight and obese women had 3.27, and 5.14 folds increase in the odds of developing GDM, respectively. There was a 17% increase in the risk of developing GDM with each 1 mg/dl increase in FG level. The cut points 84.5 mg/dl for FG (with 72.9% sensitivity, 74.4% specificity), 25.4 kg/m2 for BMI (with 68.9% sen-sitivity, 62.8% specificity), and 111.2 for BMI+FG (with 70.7% sensitivity, 80.6% specificity) was detected.Conclusion: On the basis of present results, since the com - bination of BMI and FG is associated with a better predic - tion value; the early screening and high -quality prenatal care should be recommended in women undergone ART with the co-occurrence of the pre-pregnancy BMI (≥25.4 kg/m2) and high FG (≥84.5 mg/dl) in the first-trimester of the pregnancy.