The Effect of Nanocurcumin and Crocin on Proliferation and Pluripotency of Bone Marrow Derived Mesenchymal Stem: Invitro Study

Human mesenchymal stem cells (hMSCs) are a multipotent cell that have self-renewal ability, immunomodulatory and neurotrophic effects,make them popular as a therapeutic method for many neurodegenerative diseases such as SCI. The use of synthetic growth factors because of their side effects have been limited to increase the survival and proliferation of MSCs. So natural extractions seem to be good alternative.Curcumin and Cronin are natural Carotenoid they are reported to have various pharmacological properties such anti-inflammatory, anti-tumor, immune and neural cell-growth stimulating effects. Therefore, we examined the effect of Nano curcumin and croc in on bone marrowmesenchymal stem cells (BM-MSCs) proliferation, pluripotency and self-renewal potential in SCI patient in an investor study. Materials and Methods: We use MTT assay and PDT test to evaluate MSC proliferation, Flow cytometry assay to measure the percentage of apoptotic MSCs. We also used Real Time PCR method to quantify mRNA expression of proliferation and pluripotency related genes OCT4 and SOX2. Results: our study demonstrated only low concentrations of Nanocurcumin and Cronin were effective for BM-MSC proliferation and increased expression level of OCT4 and SOX2 in treatment groups while high concentrations decreased survival of MSCs and caused to apoptosis compared with untreated group. Conclusion: The results may indicate the beneficial role of Nanocurcumin and crocin in the enhancement proliferation capacity of MSCs and increase pluripotency potential of them via effect on level expression of related genes, although nanocurcumin seem to be more effective than crocin.so this study suggests that using nanocurcumin and crocin separately in culturing of MSCs as an alternative for synthetic growth factors may be useful for cell therapy procedures in SCI patients.