Synthesis and Application of a Novel Magnetic SBA-15/GO@dual-Template Imprinted Polymer for Soild Phase Micro Extraction and Determination of Nortriptilyne and Amitriptyline in the Blood of Rat Plasma

Tricyclic antidepressant drugs (TCAs) have unique ability for treatment depressive states and other psychiatric disorders [1]. Therapeutic drug monitoring is highly recommended to achieve best therapeutic concentration with at least overdose and adverse problems. In SPE, nano-scale based sorbent lead to more improvement in method characteristics performance, due to their advantages such as higher surface area and number of reactive sites [2]. Chemometrics is a discipline within Chemistry, which allows extracting chemically relevant information via experiment optimization, data processing, calibration, quality control and organisation of the analytical process. Statistical design of experiments (DOE) is commonly seen as an essential part of chemometrics. However, it is often overlooked in chemometric practice [3]. The purpose of DOE is to provide a systematic and intelligent action plan in order to obtain the maximum possible information from the minimum experiments. Among the experimental designs, Box- Behnken Design (BBD) is one of the most effective and most practical designs [4]. In this present study, a novel dual-templated molecularly imprinted polymer (DMIP) was prepared using magnetic mesoporous silica SBA-15 modified with graphene Oxide (GO), as carrier for selective recognition and preconcentrated of trace amount of nortriptyline (NOR) and amitriptyline (AMT) in the blood of rat plasma. The resulting polymers were characterized by SEM, TEM, FT-IR, XRD, TGA, VSM and BET techniques. The results suggested a highly ordered mesoporous nanostructure anchoring of Fe3O4 nanoparticles and that the imprinted polymer was coated on the Fe3O4@SBA-15/GO surface. In this research, multivariate optimization techniques are used and influence of parameters and their interaction in extractions and sensitive determination of drugs were explored in detail through Response Surface Methodology (RSM) based on the Box–Behnken design. This technique provides a low cost and effective path to find the best laboratory conditions. Fe3O4@SBA-15/GO/MIP was successfully used as the solid-phase micro extraction (SPME) sorbent coupled with HPLC technique. Under the optimized conditions, the limits of detection (LODs) and quantitation (LOQs) of the proposed method for NOR and AMT were in the range of 0.63-0.75 ng·mL¹ and 2.1-2.5 ng·mL¹, respectively. The recoveries of NOR and AMT were obtained between 93.4% and 104.3% with relative standard deviations (RSDs) in the range of 3.3-4.2%. These resluts highlighted the good application prospect of the multi/dual-template imprinting strategy could be used as an efficient SPME adsorbent for enrichment of NOR and AMT in the blood of rat plasma.