Reperfusion reduce DNA damage and initiate DNA repairmen in testicular torsion-induced rats

Background: It has been illustrated that, the testicular torsion (TT) negatively effects testicular tissue, majorly by initiating hypoxia condition. The TT results in compeletly infertility due to a duration and degree manner. In line with current menace, the reperfusion surgery is known as the only emergency intervention to treat this syndrome. Therefore, the present study tried to show the DNA fragmentation ratio and p53 gene expression before and after reperfusion surgery. Methods: In the present study, 30 mature male Wistar rats (NO=6 rats in each group) were used. Following 4 hours from torsion induction, the reperfusion was induced. Then, the animals were subdivided into; 4 hours torsion-induced (T1), (b) 1hour post-reperfusion (T2), 2 hours post-reperfusion (T3), 4 hours post reperfusion (T4) and 8 hours post-reperfusion (T5) groups. The testicular DNA fragmentation was analyzed using DNA ladder and TUNEL test. Finally, the P53+ cell numbers per mm2 of tissue was assessed using immunohistochemical staining. Results: Observations demonstrated that, the reperfusion (albeit after 8 hours in T5 group) significantly (P<0.05) up-regulated the P53 expressions versus T1 group. Moreover, the animals in T5 group showed diminished DNA fragmentation ratio versus T1 group. In conclusion: Minimum 8 hours, post-reperfusion is needed to re-initiate necessary expressions of p53 to ameliorate torsion-induced DNA damage, avoid cellular apoptosis and restore cell cycling machinery.

Keywords:

Torsion , Reperfusion , DNA Damage , P53 , Rat