Curcumin-Loaded Mesoporous Silica Nanoparticles/Polycaprolactone-Gelatin Nanofiber Composites Enhanced hTERT Gene Expression in Human Adipose Derived-Stem Cells

Publish Year: 1398
نوع سند: مقاله کنفرانسی
زبان: English
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SRMMED22_446

تاریخ نمایه سازی: 19 آبان 1398

Abstract:

Background and Aim: This study aimed to develop a drug carrier based on amine-functionalized mesoporous silica nanoparticles (MSNs) loaded into polymeric nanofibers for efficient and sustained delivery of a poorly water-soluble drug, curcumin, and to study its effects on human telomerase reverse transcriptase (hTERT) expression levels as a hallmark of aging in human adipose tissue-derived stem cells (hADSC). Methods: For this purpose, Curcumin-loaded MSNs /Polycaprolactone-Gelatin nanofiber composites were successfully fabricated by optimizing the electrospinning parameters and characterized using FE-SEM, TEM, FTIR and UTM. hADSCs were isolated from adipose tissue and their viabilities on Curcumin-MSNs loaded into Polycaprolactone-Gelatin nanofiber were assessed by MTT. hTERT expression and percentage of senescent cells were evaluated using real-time PCR and senescence-associated β-galactosidase (SA-ß-gal) activity, respectively. Results: The results demonstrated significant proliferation of hADSCs on Curcumin-MSNs loaded Polycaprolactone-Gelatin nanofiber after 14 and 28 days compared to Curcumin loaded Polycaprolactone-Gelatin and neat Polycaprolactone-Gelatin nanofibers. In addition to, SA-ß-gal staining showed that Curcumin-MSNs loaded Polycaprolactone-Gelatin nanofiber meaningfully reduced the number of senescent cells after 14 and 28 days. Moreover, hTERT expression levels significantly enhanced in the cells seeded on Curcumin-MSNs loaded Polycaprolactone-Gelatin nanofiber than Curcumin loaded Polycaprolactone-Gelatin and neat Polycaprolactone-Gelatin nanofibers group (P<0.001). Conclusion: As a conclusion, Curcumin loaded amine-functionalized mesoporous silica nanoparticles embed in polymeric nanofibers may have an advantageous potential to enhance the lifespan of hADSCs through promoting hTERT gene expression.

Authors

Samira Mashayekhi

Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

Mohammad Pourhassan-Moghaddam

Department of Medical Biotechnology, Tabriz University of Medical Sciences, Tabriz, Iran

Maryam Ghobeh

Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran