Antibiotic Resistance and Molecular Detection of the Virulence Factors of uropathogenic Escherichia coli Isolated from Fecal and Urinary Tract Infected Patients

Publish Year: 1398
نوع سند: مقاله کنفرانسی
زبان: English
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ICCM13_113

تاریخ نمایه سازی: 25 آبان 1398

Abstract:

Background and Objectives: Nosocomial infections are acquired during hospital treatment or in a hospital environment. One such infective agent is Escherichia coli, and many virulence genes enable it to become pathogenic, causing damage to the host. This study aimed to identify some important virulence factors, including aer ،traT and PAI genes, typical of uropathogenic Escherichia coli (UPEC) in the isolates collected from fecal and urinary tract infected patients in a center in Iran by multiplex polymerase chain reaction (PCR) assay. Materials and Methods: In doing so, 75 isolates of E.coli from the urine of inpatients and 75 isolates from Commensal faecal without UTI and diarrhea were collected. Antibiotic susceptibility pattern for 14 antibiotics was done. The occurrence of three virulence genes among all the isolates was confirmed by multiplex PCR.Results: The prevalence rates of aer ،traT and PAI genes in the fecal isolates were 92%, 90.6% and 46.6%, respectively and the rates in urine isolates were 96%, 97.3% and 41.3%, respectively. Conclusion: Despite the high frequency of these genes in both populations, there was no statistical relationship between them. The most antibiotic resistance was found in AMP, CZ and SXT. These results indicate the effect of external environment (hospital setting) on host susceptibility (increase of infection) in the fecal E. coli isolates, so making the patient prone to sepsis.

Authors

Mohamad Hossein Rezaei

Cellular and Molecular Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran

Farshad Kakian

Department of Bacteriology & Virology, School of medicine, Shiraz University of Medical Sciences, Shiraz, Iran

Abolfazl Gholipour

Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran

Behnam Zamanzad

Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran