Achromobacter xylosoxidans Genomic Characterization and DNA Profiles of Strains Isolated from Blood Cultures

Publish Year: 1398
نوع سند: مقاله کنفرانسی
زبان: English
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ICCM13_210

تاریخ نمایه سازی: 25 آبان 1398

Abstract:

Introduction and objectives: Achromobacteria are ubiquitous, aerobic, non-fermentative, gram-negative rods in which misidentification is common, hindering assessment of clinical impact, and it has become essential to have a reliable and specific technique for accurate identification. It is a nosocomial pathogen and various sources like disinfectant solutions, saline etc. have been implicated in the spread of infection. In this study we applied a specific PCR for identification of this organism and assessed A. xylosoxidans genome variability by randomly amplified polymorphic DNA (RAPD). Materials and methods: A total of 70 Achromobacter isolates were collected from the blood cultures of hospitalized patients in Shiraz Namazi hospital and were analyzed in Professor Alborzi Clinical Microbiology Research Center. The strain’s species were confirmed using biochemical tests, Phoenix automated microbiology (BD, US) and API 20 NE systems (biomerieux. France) and a specific combination PCR based on the detection of blaOXA-114-like and 16S rRNA genes. All of the strains were characterized for their genomic asset by the (RAPD) technique, useful to look for genetic relationships among the isolates and the dendrogram was generated with GelJ v02 using a Euclidean distance dissimilarity matrix and the agglomeration method of Ward. All of the isolates were tested for their susceptibility against a panel of antibiotics. The antimicrobial susceptibility of the isolates was determined by disc diffusion assay following CLSI guidelines. Results: All of the isolates were identified as A. xylosoxidance by Species-specific PCR. The 70 strains of A. xylosoxidans were characterized by RAPD-PCR, useful to look for genetic relationships among the isolated strains. We showed that 7 clusters were visible based upon a threshold set at 75% of similarity. The largest cluster had 32 isolates, followed by one with 13 isolates. Five types were limited to 9 and 7, 5, 4 and 3 isolates each, respectively. All of the isolates were resistant to aztreonam, ticarcillin, ampicillin, cefalexine, cefuroxime, cefotaxime, ceftriaxone and cefixime. More than 97% of the isolates were resistant to aminoglycosides. No resistance was encountered against tigecycline, polymixin B, trimethoprim/sulfamethoxazole and ceftizoxime, Conclusion: Most isolates were resistant to aminoglycosides. All of the isolates showed variable resistance to and first- and second and third generation cephalosporins. This study performed to gain more comprehensive knowledge about the involvement of A. xylosoxidans in nosocomial blood stream infection.

Authors

Bahman Pourabbas

Professor Alborzi Clinical Microbiology Research Center, Namazi Hospital, Shiraz University of Medical Sciences, Shiraz, Iran

Shima Sepehrpour

Professor Alborzi Clinical Microbiology Research Center, Namazi Hospital, Shiraz University of Medical Sciences, Shiraz, Iran

Negin Keihani

Professor Alborzi Clinical Microbiology Research Center, Namazi Hospital, Shiraz University of Medical Sciences, Shiraz, Iran