Morphine and kisspeptin influences on 5-α reductase and aromatase gene expression in adult male rats

Publish Year: 1398
نوع سند: مقاله ژورنالی
زبان: English
View: 312

This Paper With 6 Page And PDF Format Ready To Download

  • Certificate
  • من نویسنده این مقاله هستم

استخراج به نرم افزارهای پژوهشی:

لینک ثابت به این Paper:

شناسه ملی سند علمی:

JR_IJBMS-22-12_013

تاریخ نمایه سازی: 4 آذر 1398

Abstract:

Objective(s): Kisspeptin and opioids are important factors for controlling GnRH/LH secretion. In present study, influences of kisspeptin or morphine were investigated on 5α- reductase or aromatase (CYP19) genes expression in the hypothalamus, testis and liver. It aimed to investigate whether kisspeptin pathway may control morphine effects on plasma concentration of testosterone.Materials and Methods: Twenty adult male rats in four groups received saline/saline, kisspeptin (1 nmol)/saline, morphine (5 mg/kg)/saline or kisspeptin/morphine respectively. Mean relative 5α-reductase and aromatase mRNA levels were determined by RT-PCR. Results: Morphine/saline injection increased significantly mean relative mRNA levels of hypothalamic 5α-reductase or aromatase compared to saline/saline. While morphine/saline did not alter mRNA levels of them compared to saline/saline group in the testis and liver. Kisspeptin/saline did not significantly decrease mean relative 5α-reductase or aromatase genes expression compared to saline/saline group in the hypothalamus, testis and liver. Injections of kisspeptin/morphine did not significantly decrease mean relative 5α-reductase or aromatase genes expression compared to morphine/saline group.Conclusion: Up-regulation of hypothalamic 5α-reductase or aromatase mRNA levels may partly induce the inhibitory effects of morphine on GnRH/LH release. Different effects of morphine on aromatase or 5α- reductase genes expression levels in the liver and testis compared to brain may be partly due to different sensitivity or functions of them to morphine used dose.

Authors

Homayoun Khazali

Department of Animal Science and Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran

Fariba Mahmoudi

Faculty of Sciences, University of Mohaghegh Ardabili, Ardabil, Iran

مراجع و منابع این Paper:

لیست زیر مراجع و منابع استفاده شده در این Paper را نمایش می دهد. این مراجع به صورت کاملا ماشینی و بر اساس هوش مصنوعی استخراج شده اند و لذا ممکن است دارای اشکالاتی باشند که به مرور زمان دقت استخراج این محتوا افزایش می یابد. مراجعی که مقالات مربوط به آنها در سیویلیکا نمایه شده و پیدا شده اند، به خود Paper لینک شده اند :
  • Schulster M, Bernie AM, Ramasamy R. The role of estradiol ...
  • Cailleux-Bounacer A, Rohmer V, Lahlou N, Lefebvre H, Roger M, ...
  • Shen M, Shi H.. Sex hormones and their receptors regulate ...
  • Poletti A, Coscarella A, Negri-Cesi P, Colciago A, Celotti F, ...
  • Turgeon JL, Waring DW. Androgen modulation of luteinizing hormone secretion ...
  • Vuong C, Van Uum SH, O Dell LE, Lutfy K, ...
  • Aloisi AM, Ceccarelli I, Fiorenzani P, Maddalena M, Rossi A, ...
  • Amini H, Ahmadiani A. In vivo evidence for an increase ...
  • Meczekalski B, Katulski K, Podfigurna-Stopa A, Czyzyk A, Genazzani AD. ...
  • Lippincott MF, Chan YM, Delaney A, Rivera-Morales D, Butler JP, ...
  • Roseweir AK, Kauffman AS, Smith JT, Guerriero KA, Morgan K, ...
  • Khazali H, Mahmoudi F, Janahmadi M. Hypothalamic Kiss1/ GPR54 genes ...
  • Mansour A, Khachaturian H, Lewis ME, Akil H, Watson S ...
  • Clarke H, Dhillo WS, Jayasena CN. Comprehensive review on kisspeptin ...
  • Bois C, Delalande C, Nurmio M, Parvinen M, Zanatta L, ...
  • Mahmoudi F, Khazali H, Janahmadi M. Morphine attenuates testosterone response ...
  • Mellon SH, Griffin LD. Neurosteroids: biochemistry and clinical significance. Trends ...
  • Killian J, Pratis K, Clifton RJ, Stanton PG, Robertson DM, ...
  • Torres JM, Ruiz E, Ortega E. Development of a quantitative ...
  • Torres JM, Ortega E. Precise quantitation of 5alpha-reductase type 1 ...
  • Torres JM, Ruiz E, Ortega E. Differential regulation of steroid ...
  • Hussain MA, Song WJ, Wolfe A. There is kisspeptin - ...
  • Szymanski L, Bakker J. Aromatase knockout mice show normal steroid-induced ...
  • Ross LA, Polotsky AJ, Kucherov A, Bradford AP, Lesh J, ...
  • Mittelman-Smith MA, Wong AM, Kathiresan AS, Micevych PE. Classical and ...
  • Iwasa T, Matsuzaki T, Yano K, Mayila Y, Irahara M. ...
  • Iwata K, Kunimura Y, Matsumoto K, Ozawa H. Effect of ...
  • نمایش کامل مراجع