Glucose Oxidase Immobilized On Novel Multifunctional Silver Dendritic Hierarchical Nanostructure: An Efficient and Robust Biocatalyst

Glucose oxidase enzyme (GOX) (oxygen 1-oxidoreductase) catalyzes the oxidation of β-D-glucose to gluconic acid. GOX has found several commercial applications including used in an automatic glucose assay kit in conjunction with catalase and chiefly in biosensors for the detection and estimation of glucose in industrial solutions and in body fluids such as blood and urine [1]. Immobilized enzymes are used as biocatalysts for analytical purposes in diagnostics and for preparative purposes in large scale industrial methods [2,3]. Immobilization can enhance the half-life, improve the stability, and increase the catalytic activity as well as recovery and reusability of an enzyme. Herein, we demonstrated synthesis of silver dendritic hierarchical nanostructure as unique and convenient support for immobilization of enzyme [4-6]. Then, an important enzyme, GOX, was immobilized on the nano carrier to produce robust biocatalysts for oxidation of β-D-glucose to gluconic acid. The biocatalyst was characterized by different analytical techniques. The results of biocatalyst systems exhibited the substantial enhancement of reactivity, reusability, and stability of GOX due to this strategy.[1-6]