DNA interaction studies a new complex of (1, 4-bis (3-aminopropyl)piperazine) Ni (II) perchlorate

For a long time, ammonia and amines have been important ligands in the chemistry of coordination. Amines and others Nitrogen-containing compounds are one of the most abundant organic molecules and as amino acid components, peptides, proteins and alkaloids are important in biochemistry. Polyamines, in both solid state and soluble state, form stable complexes [1]. In this study, the new complex of (1, 4-bis (3-aminopropyl) piperazine)-perchlorato-Ni (II) perchlorate was synthesized (Fig 1). Binding interaction of this complex with calf thymus (CT) DNA was investigated by emission, absorption, FT-IR, measurement techniques. Upon addition of CT-DNA, changes were observed in the characteristic UV-Vis bands of the complex. It can be observed that with increasing amounts of Ni(II) complex added to the DNA solution the intensity of the absorption peak of DNA obviously decreased (hypochromism), and complex binds to CT-DNA in an intercalative mode [2] (Fig 2).