Physiological Hyperglycemia and Hyperinsulinemia Induces Transcription of MEDA-4 and MEDA-7 in Pancrease of Rat

Introduction: Mesenteric Estrogen Dependent Adipose Gene 4 and 7 (MEDA-4 and MEDA-7) are novel adipocytikines that are expressed in pancreas, induce adipocyte differentiation and over-expressed in adipose tissue of obese animals. This study was designed to determine the effect of hyperglycemia and hyperinsulinemia on pancreatic expression of MEDA-4 and MEDA-7 in rat.Materials & Methods: In this experimental study 15 rats were divided in three groups including hyperglycemic (HG), hyperinsulinemic (HI) and control groups. HG rats received daily intraperitoneal injection of 20% glucose for 7 days, while HI animals received intraperitoneal injection of Mixtrad insulin (0.8 IU/kg) for 7 days. same volume of normal saline was injected to control animals. Serum insulin and glucose were measured 7 days after glucose and insulin treatment. Transcription levels of MEDA-4 and MEDA-7 mRNA were determined in pancrease of euthanized animals using real time PCR analysis.Results: Our results showed that hyperglycemia and hyperinsulinemia resulted in upregulation of MEDA-4 and MEDA-7 genes in pancreas. Glucose and insulin concentrations showed positive correlation with RNA levels of MEDA-4 and MEDA-7 genes.Conclusion: Induction of mRNA expression of MEDA-4 and MEDA-7 in the presence of pathological levels of glucose and insulin confirmed that these novel hormones may act as a mediator of pancreatic dysfunction in diabetes states.