Z. Ghobadi Nejad - Department of Natural Resources and Environment, Science and Research Branch, Islamic Azad University, Tehran
S. M. Borghei - Department of Natural Resources and Environment, Science and Research Branch, Islamic Azad University, Tehran ; Biochemical and Bioenvironmental Research Centre, Sharif University of Technology, Tehran
S. Yaghmaei - Biochemical and Bioenvironmental Research Centre, Sharif University of Technology, Tehran ; Department of Chemical and Petroleum Engineering, Sharif University of Technology, Tehran

The current study includes the screening of indigenous extracellular laccase producing fungus from the soil, identified as Phanerochaete chrysosporiumspecies.The selected strain was applied under the optimized condition for the production of  laccase in the solid state fermentation. The effect of polyethylene glycol and Triton X-100 on the enhancement of enzyme activity and biocatalytic removal of bisphenol A using laccase produced by Phanerochaete chrysosporium was investigated at 40°C, initial laccase activity 3U/mL and initial bisphenol A concentration 2 mM after 3 h of treatment. Bisphenol A concentration was evaluated using a colorimetric assay in the presence of 4-AAP. The effect of PEG molecular weight on BPA removal was determined using PEG with a molecular weight between 1500 and 20000.The results showed that the addition of polyethylene glycol and Triton X-100 to the reaction mixture greatly increased the laccase activity and the residual enzyme activity, but no significant changes were found in the removal of bisphenol A. The maximum removal of BPA was obtained at pH 8.0 and 40° C in 90 mg/L of PEG-1500 and 50, 100 mg/L of PEG-6000, while laccase activity at all concentrations of PEG-1500 and 6000 increased.

Phanerochaete chrysosporium, Laccase, Bisphenol A, polyethylene glycol, Triton X-100