Comparison of immunoregulatory and proliferative effects between St. john’s wort extract of callus culture and field-growing plant on adipose-derived mesenchymal stem cells from Multiple sclerosis patients

Publish Year: 1398
نوع سند: مقاله کنفرانسی
زبان: English
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شناسه ملی سند علمی:

MSC16_030

تاریخ نمایه سازی: 11 آذر 1398

Abstract:

Background: Multiple sclerosis (MS) is an inflammatory autoimmune central nervous system (CNS) disorder causing myelin sheet and axonal degeneration. Currently, pathogenesis of MS is unclear and there is no genuine therapy for this. Nowadays, Mesenchymal Stem Cells (MSCs) have attracted great interest because of immunomodulatory effects in a MS disease. Hypericum perforatum (HP) or st.john’s wort is a pharmaceutical plant with anti-inflammatory properties which plant Tissue culture as a technique could improve its pharmacological profile. Due to evaluate therapeutic potential of (HP) extract, plant callus (mass of plant parenchyma cell derived from tissue culture) extract exhibited some different anti-inflammatory compounds which are not exist in main plants. The effects of these extracts on MSCs were investigated which extract has more anti-inflammatory properties. Material & method: Adipose tissue mesenchymal stem cells (AT-MSCs) from MS patients, cultured in 6-wells plate and treated with 0,5and 10μg/ml extracts for 24h also control group was remained untreated. Proliferation was measured with MTT assay and flow cytometry analysis was assessed to measure Tregs-cell populations. Result: In This study, we observed a significantly higher percentage of Tregs cells in callus extract than plant extract. Our data showed that in 10 μg/ml extracts, callus increased Tregscell population about 3 fold and proliferation ratio about 1.5 fold more than main plant. Also there is positive correlation between increasing of both extracts concentrations and population of Tregscell. In the same concentration, callus extract is more effective than plant extract.Conclusion: This results indicated that high concentration of callus extract could be a candidate for use in MS patients who under MSCs therapy.

Authors

Negin Afsharzadeh

Research Center for Plant Sciences, Ferdowsi University of Mashhad, Mashhad, Iran

Fahimeh Lavi Arab

Immunology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran

Mahmoud Mahmoudi

Department of Immunology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

Mojtaba Sankian

Immunobiochemistry laboratory, Immunology Research Center, Bu-Ali Research Institute, Mashhad, Iran.

Leila Samiei

Research Center for Plant Sciences, Ferdowsi University of Mashhad, Mashhad, Iran

Karim Nikkhah

Department of Neurology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran