IMMUNOREGULATORY, PROLIFERATIVE AND ANTI-OXIDANT EFFECTS OF NANOCURCUMINOIDS ON ADIPOSE-DERIVED MESENCHYMAL STEM CELLS DERIVED MULTIPLE SCLEROSIS

Introduction Multiple sclerosis (MS) is an inflammatory mediated demyelinating disease of the human central nervous system. Conventional disease modifying therapy are not cure for progressive phase of multiple sclerosis. Stem cell therapy as a new therapeutic approach also has attracted great interest. Curcuminoids are popular for their pleiotropic therapeutic functions, such as their anti-inflammatory and anti-oxidant effects. Curcuminoids can enhance usability of other drugs such as stem cell therapy for MS. Nonetheless, their clinical use is associated with poor systemic bioavailability and insolubility. The nano-formulation of curcuminoids eliminates these shortcomings. Here we have examined the effect of nano micelle form of Curcuminoids on adipose-derived mesenchymal stem cells from multiple sclerosis patients.Material and Methods: In the present study, we explored immunoregulatory, proliferative and anti-oxidant effects of nanocurcuminoids on adipose-derived mesenchymal stem cells (AT-MSCs) from multiple sclerosis patients. Flow cytometry analysis and MTT assay were employed to explore the effects of nanocurcuminoids on the apoptosis and proliferation of adipose-derived MSCs (ATMSCs). The anti-oxidant effect of nanocurcuminoids on AT-MSCs also was examined. The immune regulatory effect of nanocurcuminoids was evaluated by the flow cytometric measurement of the T regulatory (Treg) population. The expression of inflammatory and anti-inflammatory cytokines was quantified using real-time PCR.Results: Our findings demonstrate that low concentrations of nanocurcuminoids are beneficial for MSC proliferation, protection of MSCs from apoptosis, reducing inflammatory cytokines and SOD activity. A high concentration of nanocurcuminoids increases the population of Tregs and elevates the expression of TGFβ and FOXP3 genes. Conclusions: The beneficial effects of nanocurcuminoids on AT-MSCs were mainly observed at low doses of nanocurcuminoids.

Keywords:

multiple sclerosis , nanocurcuminoids , adipose-derived mesenchymal stem cells