The Cytotoxic Properties and Anticancer Impacts of Zinc oxide Nanoparticles On Human Breast, MCF7 Cell Lines

Nanotechnology enable us to manipulate the substances at nanoscale level. Metallic nanomaterials such as Zn O nanoparticles synthesized with chemical methods, which has the disadvantage of using deleterious chemicals. Green chemistry allows the design and synthesis of nanoparticles, with reducing or elimination of hazardous materials. One of the important applications of zinc oxide nanoparticles is to use as an antibacterial and anticancer agent. In this study, we synthesized Zn O nanoparticle using Hyssopus officinalis plant extract and evaluate its their cytotoxicity on MCF7 breast cancer cell line. Materials and Methods: The MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) cell viability assay was done on MCF7 cell line. Cells were seeded on 96-well plates and incubated for 24 hours. After 24 hours, cells were treated with different concentrations of Zn O nanoparticles (8, 16, 32, 64 and 128 μg / ml) and incubated for next 24, 48 and 72 hours. Then, treatments removed and MTT was added to each well. DMSO was used in order to dissolve insoluble blue crystals and absorbance was read at 570 nm. Results: Results showed that Zn O nanoparticles synthesized in Hyssopus officinalis plant extract are able to inhibit MCF7 cells. However, this inhibition was at low concentrations of Zn O nanoparticles and the values of IC50 (in 24, 48 and 72-hour treatment) respectively was calculated at 16, 10 and 5 μg/ml NPs. Conclusion: Results of this study indicated the inhibitory effects of Zn O nanoparticles synthesized in Hyssopus officinalis plant extract on cancer cells. However, in vitro studies should perform in order to confirm these effects. Finally, these results demonstrate the anticancer potential of Zn O nanoparticles, which could be used as therapeutic agent in cancer therapy.

Keywords:

Green Chemistry , Zn O Nanoparticles , Hyssopus Officinalis , MCF7 Cell Line , Breast Cancer..