Background: In recent years, the genus
Asaia has been isolated from different insects such as: Anopheles, Culex and Aedes. This bacterium has unique features such as presence in different organs of mosquitoes (midgut, salivary glands and reproductive organs) of female and male mosquitoes and vertical and horizontal transmission. These specifications lead to the possibility of introducing
Asaia as a robust candidate for different control of vector borne diseases via paratransgenesis technology. Several studies have been performed on the microbiota of
Aedes mosquitoes in Iran and the Middle East to find a suitable candidate for controlling the malaria based on paratransgenesis approaches. The present study is the first report of isolation and characterization of the genus
Asaia within
Aedes aegypti species which originated from southeast and east of Iran.Objectives: The main purpose of this study is isolation, biochemical and molecular characterization of the genus
Asaia within
Aedes aegypti species originated from different part of the Iran. Materials and Methods: Mosquitoes originated from field-collected and laboratory-reared colonies of
Aedes aegypti species. Adult mosquitoes were anesthetized; their midguts were isolated by dissection, followed by grinding the midgut contents which were then cultured in enrichment broth media and later in CaCO3 agar plates separately. Morphological, biochemical and physiological characterization were carried out after the appearance of colonies. For molecular confirmation, selected colonies were cultured, their DNAs were extracted and PCR was performed on the 16S ribosomal RNA.Results: In this study, morphological, biochemical, physiological and molecular results indicated that all isolates are members of the genus
Asaia bacteria. Bacterium were identifiedin the middle midgut of the
Aedes aegypti mosquitoes by using the culture dependent method and sequencing the 16s rRNA gene.Conclusion: Our findings show that
Asaia bacteria are present in both insectary-reared colonies and field-collected mosquitoes and can be isolated by simple and specific methods. Furthermore, with respect to the fact that we isolated
Asaia within the
Aedes aegypti specimens from distinct climatic and zoogeographical regions, it is promising and may be concluded that species of this genus can tolerate the complicated environmental conditions of the vector-borne diseases endemic regions. Therefore, it can be considered as a promising target in paratransgenesis and vector control programs.