A.Pourbabaee - Department of Biology, Faculty of Science
F.Najafi - Institue of Biochemistry and Biophysics, University of Tehran
M.N.Sarbolouki - Insititute of Biochemistry and Biophysics, University of Tehran
F.Malekzadeh - Departman of Biology Faculty of Sciecne

Among the nwenty bacterial strains isolated from the effluent water of a textile mill in central Iran, one was able to decolorize Secrilene Black (Disperse Black) dye, (range : 10-100 mg/l), present in artificial or real effluents. Biolchemical characterization indicated that the strain is an aerobic strain of Bacillus sp. Studies via NMR and FT.IR and GC-MS showed that decolorization occurs by viable and active cells within 30 min (cell biomass / dye: 5/1) without any need for extra carbon of nitrogen sources, whereas biodegradation takes place in 7 days. Studies showed that bioremediation occurs via a mechanism that is heretofore unreported , eg unaffected by inhibitors like menadione, sodium azide, di-nitrophenol and jodosobenzoic acid, products of biodegradation are non-toxic against the PS bacteria, juman cells and Phanerochate chrysosporium (PTCC 5130) . It is thus concluded that SP strain could be advantageously utilized for the treatment of effluents containing Serilene Black dye.