TISSUE CHANGES FOLLOWING METHAMPHETAMINE USE IN ADIPOSE TISSUE OF EXPERIMENTAL RATS: BEHAVIORAL AND PATHOLOGICAL ASSESSMENT

Publish Year: 1398
نوع سند: مقاله کنفرانسی
زبان: English
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KAMED13_163

تاریخ نمایه سازی: 10 دی 1398

Abstract:

Background and Aim : Methamphetamine (METH) abuse is considered as a major health concern leading to agitation, hyperkinesia, hyperthermia, and even death, affecting globally 24.7 million populations. This study was carried out to assess the behavioral modifications and pathological changes in adipose tissue after METH use in experimental rat model.Methods : .5 mL/rat) for 3 weeks, group 2 was the sham group receiving normal saline (0.5 mL/rat, ip) and the 3rd group was the Thirty male Wistar rats were randomly allocated to three equal groups. Group 1 was experimental receiving METH (2 mg/kg, ip, 0control receiving distilled water, identically. Elevated plus-maze (EPM) using a plus maze, evaluated the animals’ behavior by ODLog program and the nociceptive sensitivity was determined by Plantar test. Histological assessment after 7, 14 and 21-days post-interventions revealed the changes. Results : Histological changes following METH use were saponification, degeneration, infiltration of mononuclear cells (MNCs), inflammation, steatites, and necrosis. Regarding EPM at the end of the first week following METH use, mean percentage of open arm time (OAT), mean percentage of time spent in the closed arm time (CAT), mean number of times rats passing from the center and the mean number of rats’ peek were significantly different showing reduction in anxiety in experimental group. After two weeks, the findings were identical except for mean number of times rats passing from the center and after 3 weeks for mean number of times rats passing from the center and mean number of rats’ peek.Conclusion : METH use resulted in degeneration, inflammation, and necrosis in adipose tissue. Based on EPM findings after 1, 2 and 3 weeks following METH use, a significant reduction of anxiety was noted in experimental group.

Authors

Fariba Jaafari sheibani

Department of Biology, Shiraz Branch, Islamic Azad University, Shiraz, Iran

Fariba Jaafari Sheybani

Associate Professor of Burn and Wound Healing Research Center, Shiraz University of Medical Sciences, Shiraz, Iran

Davood Mehrabani

Department of Biology, Shiraz Branch, Islamic Azad University, Shiraz, Iran

Seyed Ebrahim Hoseini

Comparative and Experimental Medicine Center, Shiraz University of Medical Sciences, Shiraz, Iran

Amin Derakhshanfar

Comparative and Experimental Medicine Center, Shiraz University of Medical Sciences, Shiraz, Iran