Mina Maftouh - Mashhad University of Medical Sciences
Malihe Hasanzadeh - Mashhad University of Medical Sciences
Hojjatolah Zamani - University of Guilan
Mehraneh Mehramiz - Mashhad University of Medical Sciences
Banafsheh Hashemi - Mashhad University of Medical Sciences
Mina Maftouh - Mashhad University of Medical Sciences

Background and Aim : Cervical-cancer is among the most commonly diagnosed cancer in woman, and the presence of HPV is reported to be related with increased inflammatory cytokines. Here we explored the association between a genetic variant in Interleukin-10 (IL-10) and cervical-cancer-risk and its relationship with IFN-γ, TGF-β, TNF-α in 350 cases with/without cervical-cancer. Methods : This case-control study comprised 146 patients with cervical-cancer and 169 healthy controls. Serum IFN-γ, TGF-β and TNF-α levels were measured, while Taq-man®‐probes‐based assay was used to determine genotypes of the polymorphism. Results : Our data showed a significant association between the AA-genotype and the presence of cervical-cancer. In particular the regression models in recessive model, showed that patients with AG/AA genotypes had a higher risk of developing cervical cancer with odds ratio (OR) of 2.85 (95% confidence interval [CI]: 1.07-7.56, p=0.03), compared with the GG genotype. Also the IFN-γ, TGF-β and TNF-α levels in cervix tumor tissue were significantly higher in patients (P<0.001) and were associated with AA genotype. Conclusion : our finding demonstrated the association of the gene variant in IL-10 gene with risk of developing cervical-cancer and higher level of inflammatory markers, indicating further investigations on the value of emerging marker for risk stratification in management of cervical cancer patients.

cervical cancer, human papillomavirus, Interleukin 10, IFN-γ, TGF-β, TNF-α