Comparison of acute and chronic experimental epileptic activity amplitude in male rats

Background and Aim : This study aimed to compare the amplitude of spikes induced by acute and chronic intraperitoneal injection of pentylenetetrazol in adult male rats.Methods : In this study, 22 adults male Wistar rats (200-250 g) were used in the control (n=6), acute (n=6) and chronic group (n=10). After anesthesia with the combination of ketamine (80 mg/kg) - xylazine (eight mg/kg), the animal s head was held fixed using a stereotaxic device and after longitudinal incision in the scalp and wiping the tissue and determining the Bregman point, the intracranial recording electrode was inserted into the CA1 hippocampal striatum layer and seizure activity was induced by intraperitoneal injection of pentylenetetrazol (80 mg/kg). After digestion, the recording electrode was inserted into the CA1 hippocampal striatum layer and seizure activity was induced by intraperitoneal injection of pentylenetetrazole (80 mg/kg). Animals were anesthetized with the combination of ketamine (80 mg/kg) - xylazine (eight mg/kg) and after fixation using a stereotaxic apparatus, followed by longitudinal incisions in the scalp and wiping the tissues on the skull, intracranial recording electrodes were placed in the CA1 hippocampal striatum layer, and seizure activity were induced by intraperitoneal injection of pentylenetetrazol (80 mg/kg). In the control group, after recording the activity in basal condition, normal saline and in the acute, group were injected intraperitoneally with pentylenetetrazole and the amplitude of spikes was evaluated by eTrace software. However, whereas in the chronic group for eight weeks on Saturday, Monday and Wednesday, 30 mg/kg pentylenetetrazole were injected intraperitoneally and after the end of this epileptic activity period, similar to the grave group. Diazepam 10 mg/kg was used to suppress epileptic activity induced by pentylenetetrazol.Results : The results of the present study showed that no spike activity was recorded in the control group and the difference of spike amplitude in the chronic group compared to the acute group was not statistically significant. Data were analyzed by SPSS software version 22 using one-way ANOVA and Tukey post hoc test. P <0.05 were considered significant.Conclusion : The causes and mechanisms involved in spike amplitude changes in favor of their reduction or elevation are unclear, and further studies are needed to clarify the issue.

Keywords:

Acute , Chronic , spike amplitude , Epilepsy , and Pentylenetetrazol