Evaluation of antiparkinsonism effects of total extract of fruits and aerial parts of Sambucus ebulus Lin 6-OH dpamine model of Parkinson in neuroblastoma cell culture

Background and Aim : Parkinson’s disease is the second most common neurodegenerative disorder which affecting the senile population with manifestation of motor disability and cognitive impairment.Sambucus ebulus is a plant with traditional uses which might confer neuroprotective effects most probably according to its anti-oxidative stress activity but there are no studies around its role in Parkinson diseases so far. Hence, this study has aimed to investigate the neuroprotective effect of total extract of fruits and aerial parts of Sambucus ebulus in a neurotoxin-induced model of Parkinson.Methods : In vitro model of Parkinson disease has generated by exposing SH-SY5Y neuroblastoma cells to neurotoxin: 6-hydroxydopamine (6-OHDA) 100μM/well.Total extract of fruits and aerial parts of Sambucus ebulus extracted by tow solvents of methanol and ethyl- acetate by maceration method. Cytotprotective effect of methanol and ethyl acetate extracts in five concentrations on cell viability by using MTT assay. Apoptotic assay was done with route of Annexin V-propidium iodide method by flow- cytometryResults : According to MTT assay analysis,both methanol and ethyl acetate extracts have shown protective effect against 6-OHDA induced cytotoxicity in SH-SY5Y neuroblastoma cells especially at concentrations of 30 and 60 μg/ml P<0.05 but apoptetic analysis has shown at IC50 Conct, only methanolic extract of the heeb had anti-apoptic effect P<0.05.Conclusion : We can introduce aerial parts of sumbacus ebulus extract as a cytoprotective co-trearment in Parkinson disease but compelementary studies especially in in vivo and clinical trials are necessary.

Keywords:

Sambucus ebulus , Apoptosis , SH-SY5Y , 6-OHDA , Neurodegenerative disorder , Parkinson’s disease , Neuroprotection