Cytotoxic and Apoptogenic Effects of Resveratrol on the Glioblastoma Cell Line

Background and Aim : Objective: Glioblastoma (GBM) is the most prevalent and aggressive primary cerebral tumor.Unfortunately, the median survival time is 15 months despite maximum treatment because the tumor is resistant to most therapeutic modalities. There are several studies indicating the chemopreventive and chemotherapeutic activity of Resveratrol as an anthocyanin component. Therefore, this study aimed to illustrate the cytotoxic and apoptogenic effects of Resveratrol in the U87 cell line (human GBM cell line).Methods : Methods: Cytotoxic activity was evaluated using an MTT assay after treatment with Resveratrol at different concentrations in the U87 cell line. Cisplatin was used as a positive control for 24 and 48 h. In addition, the percentage of apoptotic cells was determined using an Annexin V/PI assay and the expression of bax, bcl2, and p53 genes was assessed using real-time PCR.Results : Results: Treatment of U87 cells with 40 μg/mL of Resveratrol resulted in 32% apoptotic cells after 24 h. To further confirm that Resveratrol treatment induced apoptosis in U87 cells, the RNA expression of bax, bcl2, and p53 genes was investigated after treatment. Real-time PCR indicated that the expression of bax and p53 increased, whereas the expression of bcl2 decreased.Conclusion : Conclusions: Resveratrol had an apoptogenic effect in the GBM cell line. This study may provide new information regarding the therapeutic effects of Resveratrol in GBM that could ultimately lead to the production of new drugs.

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Keywords: Glioblastoma , U87 Cells , Cisplatin , Resveratrol , Apoptosis