The adipose-derived stem cells therapy by magnetic attraction in a rat model of Parkinson’s disease

Background and Aim : Abstract Background: Stem cell therapies for neurodegenerative diseases such as spinal cord injury, stroke, and Parkinson’s disease (PD) are intended to replace lost neuron. The major point of this treatment is to guide the migration of transplanted cells into the injury site. In this, study the homing of super paramagnetic iron oxide nanoparticles (SPIONs) labeled adipose-derived stem cells (ADSC) by an external magnetic (EM) field were investigated in a rat model of PD.Methods : Material & method: ADSCs were obtained from perinephric regions and then ADSC markers were assessed by immunostaining with CD90, CD105, CD49d, and CD45. The SPION was coated using poly- L- lysine hydro bromide and labeled ADSC and then transfected by the GFP reporter gene. Rats with PD were divided into five groups: a intact group, a control group with PD (lesion with 6-HD injection), and three experimental groups: the PD/ADSC group (PD transplant with ADSCs labeled by BrdU), PD/ADSC/SPION group (PD transplant with ADSCs labeled with SPION and transfected by GFP); and the PD/ ADSC/ SPION/ EM group (PD transplant with ADSCs labeled with SPION and transfected by GFP induced with external magnet).Results : Abstract Background: Stem cell therapies for neurodegenerative diseases such as spinal cord injury, stroke, and Parkinson’s disease (PD) are intended to replace lost neuron. The major point of this treatment is to guide the migration of transplanted cells into the injury site. In this, study the homing of super paramagnetic iron oxide nanoparticles (SPIONs) labeled adipose-derived stem cells (ADSC) by an external magnetic (EM) field were investigated in a rat model of PD. Material & method: ADSCs were obtained from perinephric regions and then ADSC markers were assessed by immunostaining with CD90, CD105, CD49d, and CD45. The SPION was coated using poly- L- lysine hydro bromide and labeled ADSC and then transfected by the GFP reporter gene. Rats with PD were divided into five groups: a intact group, a control group with PD (lesion with 6-HD injection), and three experimental groups: the PD/ADSC group (PD transplant with ADSCs labeled by BrdU), PD/ADSC/SPION group (PD transplant with ADSCs labeled with SPION and transfected by GFP); and the PD/ ADSC/ SPION/ EM group (PD transplant with ADSCs labeled with SPION and transfected by GFP induced with external magnet). Result: ADSCs were immunoreactive to fat markers CD105 (89.4±0.9), CD90 (88.93±1.1), and CD49d (75.6±0.6), with negative immunostaining at the hematopoietic stem cell marker (CD45: 1.9±0.8). The highest number of GFP-positive cells was in the ADSC/SPION/EM group (59.7±1.1), which was significantly different from that in ADSC/SPION group (38.07±2and p≤0.008).Conclusion : Conclusion: External magnets can promote delivery and homing of transplanted stem cells in injury site.

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Keywords: Super Paramagnetic Iron Oxide Nanoparticles (SPIONs) , adipose derived stem cells (ADSC) , Parkinson s disease