Association of paraoxonse1 (PON1) genotypes with the activity of PON1 in patients with Parkinson′s disease

Background and Aim : Parkinson s disease (PD) is defined as a chronic neurodegenerative disorder. A wide range of factors, such as oxidative stress, neurotoxins, and pesticides, can affect Parkinson′s disease (PD). In this study, we surveyed the relationship between PON1 polymorphisms and Parkinson’s disease pathogenesis.Methods : We investigated two polymorphisms (L55M and Q192R) of the PON1 gene by PCR-RFLP assays. We also measured the levels of PON1, TAC (total antioxidant capacity), and TOS (total oxidant status) with ELISA (Enzyme-linked immunosorbent assay) and spectrophotometric methods.Results : Paraoxonase and arylesterase activity of PON1 as well as their concentrations were lower in patients with PD compared to control group; however, this difference was not significant. The levels of TOS and OSI were higher in patients group, while the levels of TAC were lower compared to control group. Serum PON1 concentration and its activity was higher in LL (in comparison with LM and MM) and RR (in comparison with QR and QQ) genotypes while we did not observe any significant differences in arylesterase levels among the mentioned polymorphisms.Conclusion : In the current study, we reported associations between PON1 polymorphisms (55, 192) and enzyme activities in Parkinson s disease. Results showed that there was a significant reduction in PON1 levels in patients with Parkinson compared with healthy samples. Taken together, paraoxonase enzyme in subjects with different genotypes could be a potential biomarker for determining the severity and prognosis of Parkinson. However, more studies are needed to be done to clarify its clinical values.

Keywords:

Parkinson s disease , Paraoxonase1 , Polymorphism