Acute buprenorphine treatment and PGC-1α gene expression during methamphetamine addiction in male rat

Publish Year: 1398
نوع سند: مقاله کنفرانسی
زبان: English
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NSCMED08_461

تاریخ نمایه سازی: 15 دی 1398

Abstract:

Background and Aim : Methamphetamine dependence (addiction) is a serious problem. Oxidative stress has shown to be a promising lead in explaining, at a cellular level, the harmful effects of MA abuse. Buprenorphine is a semisynthetic opioid derived from thebaine, a naturally occurring alkaloid of the opium poppy, Papaver somniferum Buprenorphine is a partial mu receptor agonist originally developed as analgesic but its potential utility for the management of opioid dependence has been discussed since early the 1970. PGC-1a is a transcriptional co-activator that can be induced by oxidative stress and coordinates the expression of multiple antioxidant programs. The primary aim of this study was therefore to investigate the alteration of PGC-1α gene following methamphetamine addiction in male rats that were under treatment of buprenorphine.Methods : 49 male Wistar rats were randomly assigned into seven experimental groups (n=7): Control, Saline, Methamphetamine (10 mg/kg, i.p. for 5 days), Buprenorphine (6 and 10 mg/kg, i.p.), Methamphetamine + Buprenorphine (6 and 10 mg/kg for 5 days). Brain stem tissue was assayed for the expression of P2X4 receptor gene using RT- PCR.Results : amphetamine administration significantly decreased the level of PGC-1a gene in comparison to control group but it was not significant. The expression of PGC-1a gene did not change after the buprenorphine (6 and 10 mg/kg) administration in comparison to control group.Conclusion : : methamphetamine toxicity influenced mitochondria function by changing the level of PGC-1a gene expression. It seems mitochondria biogenesis gene play a role in methamphetamine toxicity.

Authors

Mobina Roshanaei Mellat

Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran.

Homeira Hatami

Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran