The effect of Lactobacillus rhamnosus on TNF α gene expression in the hippocampus of rat in LPS-induced inflammation model

Publish Year: 1398
نوع سند: مقاله کنفرانسی
زبان: English
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NSCMED08_577

تاریخ نمایه سازی: 15 دی 1398

Abstract:

Background and Aim : Bacterial lipopolysaccharide (LPS) as a structural part of outer membrane of Gram-negative bacteria which can activate monocytes, macrophages and releasing pro-inflammatory cytokines such as TNF-α, IL-1β and IL-6. Increased levels of pro-inflammatory cytokines and overproduction of free radicals can associate with neuro-inflammation, neural death, memory deficit and finally pathogenesis of neurodegenerative diseases such as Alzheimer. Probiotics defined as live microorganisms which, when administered in adequate amounts, can confer health benefits to the host. when probiotics be administered, these bacteria can be colonized permanently or transitionally by passing through the gut. The relation of the gut microbiota effects on behavior and cognition are increasingly investigating. There is a bidirectional pathway between the gut microbiota and the central nervous system that compromise enteric and autonomic nervous systems (ENS, ANS), neuroendocrine signaling pathway and neuro-immune system. Gut microbiome produce the variety components such as neurotransmitters and neuromodulators or can affect the production of them by the host. The relation between the gut microbiota and the brain has established a new biological pathway which called microbiota-gut-brain axis. Recent researches have indicated the impacts of this pathway in prevention and treatment of nervous system diseases. In the present study, the potentially effect of probiotics on the amelioration of the inflammation induced by LPS has investigated.Methods : rats were divided into four groups: 1-Control (gavage with saline), 2-Lactobacillus rhamnosus PTTC1637 (gavage), 3-treated with LPS (intraperitoneal injection), 4-Lactobacillus rhamnosus PTTC1637+LPS. Gavage was performed for 21 days. Finally, after the treatments were completed, the hippocampi were isolated and quantification of TNF-α gene expression was performed by Real Time PCR.Results : LPS-induced inflammation led to the significant increased level of TNF-α gene expression in the hippocampus. Treatment with Lactobacillus rhamnosus PTTC1637 decreased the level of TNF-α gene expression in the hippocampus, significantly.Conclusion : The level of TNF-α gene expression has a direct relation with inflammation and inflammation can be associated with neurodegenerative diseases. Probiotics as beneficial microorganisms which can colonize in the gut and produce different components, can influence the physiological functions in a positive way. Probably, probiotics by decreasing TNF-α mRNA expression and subsequently inflammation, can influence the different aspects of behavior and cognition.

Authors

Sayyed Iman Zolfaghari

Department of Biology, Faculty of Science, University of Isfahan, Isfahan, Iran

Mohammad Rabbani Khorasgani

Department of Biology, Faculty of Science, University of Isfahan, Isfahan, Iran

Maryam Noorbakhshnia

Department of Biology, Faculty of Science, University of Isfahan, Isfahan, Iran