The effect of Glutamine on serum levels of glyoxal and methylglyoxal in type 2 diabetic rats

Glyoxal and methylglyoxal are mediated by glycolytic agents and contribute to vascular complications in diabetes. The levels of glyoxal and methylglyoxal, enhance in diabetes. Regarding the role of glycemic products in the development of diabetes and the reduction of the level of glutamine in diabetic plasma, the aim of this study was to measure the effect of glutamine on glyoxal and methylglyoxal in type 2 diabetic rats.Materials & Methods: 30 male Wistar rats were divided into 3 groups: 1) healthy group 2) diabetic group receiving peritoneal injection of 110 mg / kg nicotinamide and 60 mg / kg streptozotocin 3) glutamine-treated diabetic group. After induction of diabetes, the rats were treated daily with oral glutamine at a dose of 1 g / kg and treated for 7 weeks. The animals were then anesthetized and blood was collected from the heart and serum was isolated and the serum glyoxal and methylglyoxal levels were measured using HPLC technique. These compounds were derived with o-phenylenediamine and determined at a wavelength of 315 nm against the standard glyoxal and methylglyoxal.Results: The results showed that glyoxal and methylglyoxal levels significantly increased in the diabetic Compared to control group Respectively (2.30 and 2.47 fold).Treatment with glutamine reduces glyoxal and methylglyoxal levels ( 5.69% and -33.57%) respectively .Conclusion & Discussion: According to the results, glutamine can be used as an anti-glycemic compound in reducing the complications of diabetes and decreasing serum glyoxal and methylglyoxal levels.

Keywords:

Type 2 Diabetes , Glutamine , Glyoxal , Methylglyoxal