Publisher of Iranian Journals and Conference Proceedings

Please waite ..
CIVILICAWe Respect the Science
Publisher of Iranian Journals and Conference Proceedings

Cloning, expression and characterization of a HER2-HER3 DNA as a novel immunotherapy agent

تعداد صفحات: 1 | تعداد نمایش خلاصه: 72 | نظرات: 0
سال انتشار: 1398
کد COI Paper: BSIPD01_052
زبان Paper: Englishglish
نسخه کامل Paper در کنفرانس ارائه نشده است و در دسترس نیست.

مشخصات نویسندگان Paper Cloning, expression and characterization of a HER2-HER3 DNA as a novel immunotherapy agent

Katayoun Heshmatzad - مرکز تحقیقات ژنومیک پزشکی، دانشگاه علوم پزشکی آزاد اسلامی تهران ایران

چکیده Paper:

Introduction: Breast cancer(BC) is one of the common disease among women worldwide and about 2.1 million new cases were diagnosed in 2018.Despite of effective treatments such as chemotherapy, radiotherapy and Hormone therapy BC has been remained one of the significant reasons of women ‘s death. Recent progress in the new field called immunotherapy has changed the management of breast cancer completely and plays a major role in the efficacy improvement of BC conventional treatments. One of the significant type of Antigen-specific immunotherapy is DNA vaccine. This type of vaccine is the result of designing an expression vector which encodes a special antigen and in the most cases this antigen is over expressed in that specific type of cancer. Cancer vaccines have led to modest effects in treatment of cancer affected patients due to immunosuppression in micro environment of Tumors. cytokine-based adjuvants such as interleukin (IL)-12 have been suggested as one of the most effective enhancing immune responses optionsMaterials & Methods: The nucleotide sequences of HER2 and HER3 and CCL20 were selected according to relevant studies. The selected sequences submitted to the Basic Local Alignment Search Tool (BLAST) to check the conservation of the selected Sequences. These sequences were linked together using a linker. The GFP tag was designed at the N terminal of the construction. ,The CMV promoter was designed and the selected sequences were designed between two restriction enzyme sitesIn this study Bioinformatics Tools and In-silico prediction methods were used in order to select the appropriate HER2, HER3, CCL20 sequences and the standard form of the recombinant Vector(PIRES2-GFP). PIRES2-GFP transfection in to HECK 293 T cell line was carried out.Results: According to previous studies and bioinformatics analysis, we selected three main sequence of HER2,3 and ccl20.24h after transfection of recombinant srtructure,green light emission was detected under fluorescence microscopeConclusion & discussion: The study results indicated that the recombinant structure has the potential to be expressed and this structure has the capacity to use in animal models in order to evaluate the immunogenicity.DNA vaccine constructions have indicated promising results and they can use seperatyely or in combination with other conventional treatments in order to improve the efficacy of the treatment.

کلیدواژه ها:

Breast cancer , DNA vaccine,HER2,HER3

کد Paper/لینک ثابت به این Paper

برای لینک دهی به این Paper می توانید از لینک زیر استفاده نمایید. این لینک همیشه ثابت است و به عنوان سند ثبت Paper در مرجع سیویلیکا مورد استفاده قرار میگیرد:

کد COI Paper: BSIPD01_052

نحوه استناد به Paper:

در صورتی که می خواهید در اثر پژوهشی خود به این Paper ارجاع دهید، به سادگی می توانید از عبارت زیر در بخش منابع و مراجع استفاده نمایید:
undefined, undefined,1398,Cloning, expression and characterization of a HER2-HER3 DNA as a novel immunotherapy agent,اولین همایش علوم پایه دربیماریهای ارثی کودکان,Tehran,,,

در داخل متن نیز هر جا که به عبارت و یا دستاوردی از این Paper اشاره شود پس از ذکر مطلب، در داخل پارانتز، مشخصات زیر نوشته می شود.
برای بار اول: (1398, Heshmatzad, Katayoun؛ )
برای بار دوم به بعد: (1398, Heshmatzad؛ )
برای آشنایی کامل با نحوه مرجع نویسی لطفا بخش راهنمای سیویلیکا (مرجع دهی) را ملاحظه نمایید.

Research Info Management

Certificate | Report Paper
این Paper در بخشهای موضوعی زیر دسته بندی شده است:
  • علوم پزشکی > سرطان
  • Export Citation info of this Paper to research management softwares

    علم سنجی و رتبه بندی Paper

    مشخصات مرکز تولید کننده این Paper به صورت زیر است:
    نوع مرکز: azad university
    تعداد مقالات: 550
    در بخش علم سنجی پایگاه سیویلیکا می توانید رتبه بندی علمی مراکز دانشگاهی و پژوهشی کشور را بر اساس آمار مقالات نمایه شده مشاهده نمایید.

    New RelatedPapers

    Share this paper


    COI is a national code dedicated to all Iranian Conference and Journal Papers. the COI of each paper can be verified online.