Preparation of Chitosan/ Polycaprolactone/ (N-Decyl-N,N-dimethyl-1- decanaminium chloride) blend Nanofibrous Tissue Engineered Constructs for Skin Tissue Regeneration

Objective: By using dual pomp electerospinning of polycaprolactone (PCL) containing N-Decyl-N, N-dimethyl-1-decanaminium chloride (DDAC) and chitosan/polyethylene oxide (PEO) the nanofibers with hydrophilic and hydrophobic properties, that can improves wound healing and accelerate its process, were achived.Approach: Prepared CS/PEO-PCL/DDAC nanofibers, which their physicochemical properties were studied, used for investigation of antibacterial properties, cytotoxicity and cell attachments and to treat full-thickness excision wound model that was created using 5mm biopsy punch in dorsal skin of Balb/c mice. Histological experiments after tissue isolation were done 3 weeks after starting treatment while wounds images analysis investigated during healing process.Results: Addition of DDAC to CS/PEO-PCL blend Nanofibrous decreased the nanofibers diameter which is a crucial property for wound healing. In spite of having proper cell adhesion, higher loading of DDAC in CS/PEO-PCL nanofibers had the most cell viability. Histology experiments showed CS/PEO-PCL nanofibers containing DDAC, had the best epithelization, most angiogenesis and lowest inflammation.Innovation: hydrophobic nanofibers absorb the hydrophobic pathological microorganisms of the wounds according to the principle of the absorption of two hydrophobic substances in an aqueous medium while the hydrophilic nanofibers, as the proper presence of micropores, increase cell adhesion and proliferation. Despite the antibacterial properties of DDAC, dual pump electrospinning of these material, with different interactions, made an innovative dressing with mentioned features.Conclusion: CS/PEO-PCL containing 8 wt. % of DDAC nanofibers are promising as a wound dressing, with both hydrophilic and hydrophobic properties, in the health care industry.

Keywords:

Wound dressing , Hydrophobic , nanofiber , Polycaprolactone , Chitosan , N-DecylN , N-dimethyl-1 decanaminium chloride (DDAC)