A Priliminary Study for Characterization of Placentas Mesenchymal Stem Cells for Application in Skin Diseases Cell therapy

Background: Recently, identification of new sources of stem cells developed, pursuing to find easily available organs and tissues, which will give rise to homogenous and effective stem cells populations. Up to present, bone marrow-derived mesenchymal stem cells are unanimously considered to fulfill the criteria for being used in clinical applications, but adipose stem cells, placenta, amnion and umbilical cord stem cells, and other tissue stem cells are making their way to being used in autologous transplantation.Materials and Methods: We isolated cellular populations from placentas stem cells using the explants and enzymatic method. The placentas-derived cells were cultured and expanded in appropriate conditions for generation of stem cells. We assessed the stem cells characteristics of the tissue-isolated cells and compared them to a MSCs line. For this purpose, we determined the immunophenotype, differential potential and morphological characteristics, as well as functional abilities placentas-derived cells. Results: Flow cytometric evaluation of cells revealed presence of CD90, CD73, and CD105 stem cells markers, while the cells were negative for CD34 and CD45. Morphological characteristics of cells exposed analogous cellular size and morphology, similar to MSCs. Placentas-derived cells confirmed their trilineage potential, being able to differentiate into adipocytes, osteoblasts, and chondrocytes in different proportions. Conclusion: We may conclude that placentas cells are endowed with characteristics of mesenchymal stem cells, and purification procedures are additionally required for safe use of these cells in diverse clinical applications.

Keywords:

Placentas Stem Cells , Mesenchymal Stem Cells , Cell Therapy