Parisa Shirvani
Mohsen Chiani
Mahsa Adibi
Zeinab Bahari
Morvarid Shafiei - Department of Microbiology, Pasteur Institute of Iran, Tehran, Iran

Introduction and objectives Methicilin Resistant Staphylococcus aureus (MRSA) is a major cause of nosocomial and community-acquired infections and represents a significant burden on the healthcare system. The formation of a biofilm, and encasement of cells in a polymer-based matrix, decreases the susceptibility to antimicrobials and immune defenses, making these infections difficult to eradicate.The goal of this study was to evaluat the effect of clindamycin eluting niosome nanoparticles against biofilm of MRSA isolates. Materials and MethodsThe twenty five clinical isolates of MRSA were collected from patients with pressure and diabetic ulcers who were referred to Loghman Hospital of Tehran- Iran. The biochemical (coagulase, Mannitol salt agar, catalase, cefoxitin disk diffusion test) and Molecular (PCR for mecA gene detection) tests were used to detect MRSA. Clindamycin eluting noisome nanoparticles were synthetized using the thin film hydration method and were characterized by X- ray diffraction, Zeta potential measurement and TEM. The effect of the Clindamycin eluting niosome nanoparticles was evaluated against one, three and five day old biofilm of S. aureus isolates using TTC and Crystal violet methods.ResultsThe round shaped clindamycin eluting niosome nanoparticles, as apperceived in the TEM images, had diameter of 50-100 nm. The clindamycin eluting niosome nanoparticles have removed one, three and five day old biofilms of MRSA at the concentrations of 125 µg/mL, 125 µg/mL and 250 µg/mL, respectively.DiscussionAntibiotics eluting noisome nanoparticles can be promising for the treatment of MRSA biofilm related infections.

Clindamycin eluting niosome nanoparticles, Methicillin Resistant S.aureus (MRSA), Biofilm