Genetic variation of high molecular weight glutenin subunits in Aegilops triuncialis accessions of Khorasan provinces

Acknowledgment about plant materials genetic diversity plays an important role in protecting and improving of crops. For this purpose, variation in high molecular weight Glutenin subunit (HMWGS) in seed protein storage of 17 accessions related to Aegilops triuncialis species collected from the Khorasan Razavi and Northern Khorasan provinces were investigated by electrophoresis of sodium dodecyl sulfate polyacrylamide gel. Based on results, a total of nine different bands were identified among these accessions and were named alphabetically. The highest frequencies were for the bands a, h and i and the lowest frequent bands were b and e. There were also eight different band patterns among studied accessions. The parameters of real allele number (Na), effective allele number (Ne), Shannon index (I) and unbiased genetic diversity (uh) in Khorasan Razavi province accessions were far more than North Khorasan province accessions. Also, the percentage of polymorphic loci in Khorasan Razavi and North Khorasan accessions were 80% and 30%, respectively.

Keywords:

Aegilops triuncialis , Genetic diversity , Glutenin subunits , HMWGS.