Nasser Pouladi, - PhD, Department of Biology, Faculty of Science, Azarbaijan Shahid Madani University, Tabriz, Iran
Mojtaba Shavali, - Department of Biology, Faculty of Science, Azarbaijan Shahid Madani University, Tabriz, Iran

Backgrounds and Objectives: Breast cancer is the main cause of death especially because of this cancer in women around the world. It is a heterogeneous and multifactorial disease. According to previous reports, polymorphism in two genes; p53, which is an important tumor suppressor gene involved in wide range of cancers, and PAI-1, which is an inhibitor protein coding plasminogen involved in progression and metastasis, has been investigated.Material and Methods: In this case-control study, 200 patients and 200 healthy women in Iranian-Azeri population were investigated. At first, genomic DNA had been extracted using high salt method, then these genes had been genotyped with tetra-ARMS PCR method. Data had been analyzed with javastat online statics package and SPSS (version 22.0). Results: Genotype distribution of these polymorphisms for p53 and PAI-1 was GG=43.5%, GC=42%, CC=14.5% and 4G/5G=70.5%, 5G/5G=18.5%, 4G/4G=11%, respectively. No association between allelic frequency and genotype, individually nor in combination have been shown. Also, there was no association between combined genotype with pathological properties of cancer.Conclusion: Our results show that polymorphism of codon 72 of p53 has a significant association with breast cancer (p value<0.023). Even though there was no association in combined genotype with breast cancer in our investigated population, further studies need to be done in bigger population and other races.

Breast cancer, P53 gene, PAI-1 gene, Polymorphism