The evaluation effects of estrogen on DLGAP5 gene expression in prostate cancer-3 (PC3) cell lines

Background and Objective: Benign Prostatic Hyperplasia (BPH) and Prostate cancer are common clinical complications in urology. Recent studies have demonstrated that estrogen receptor signaling pathways may be important in the development and maintenance of BPH and Prostate cancer. Estrogen and Selective Estrogen Receptor Modulators (SERMs) have shown a potential role in promoting or inhibiting prostate proliferation in BPH and Prostate cancer. The aim of this study is to investigate the effect of estrogen on DLGAP5 gene expressions in Prostate Cancer-3 (PC3) cell lines.Materials and Methods: In this study, prostate cancer cells of the PC3 cell line were cultured in DMEM medium with 10% bovine serum. Cells were treated at 24, 48 and 72 hours intervals. The cells were exposed to concentrations of 100 nM of Estrogen hormone. Then expressions of DLGAP5 genes in cells exposed to 100 nM Estrogen hormone concentration were investigated by RT-Real Time PCR.Results: By increasing the concentration of Estrogen hormone, and time-dependently, while the expression level of DLGAP5 gene decreased. The maximum Effect was gained by 100 nM of Estrogen hormone and 72 hours after treatment of the cells.Conclusion: Estrogen hormone, in a time-dependent manner reduces the expression of the gene examined in PC3 cells. According to the current evidence, Estrogen hormone can act as an anti-cancer agent.

Keywords:

Estrogen hormone , Prostate cancer , PC3 cell line , DLGAP5 gene.