The investigation of rs174547 in FADS1 gene, rs1260326 in GCKR gene variation, for identification of related to high triglyceride

Evaluation of blood fat because of the association with certain diseases in the medical community has always been important. Blood fat including cholesterol, LDL (low-density lipoprotein), HDL (high-density lipoprotein), triglycerides. Triglycerides are an important source of energy is generally more people on calorie intake from protein used. High triglycerides can block blood vessels and damage the pancreas. Additional triglyceride tissue also has effect on metabolic diseases, including fatty liver, type 2 diabetes and some cardiomyopathies.Among the factor involved in the regulation of triglyceride metabolism can be pointed to genetic factors and environmental factors. Both of genetic mutation and environmental factor (diet, physical activity,having a high-carbohydrate dietand more) might effects on Increase of triglyceridelevel. The objective of this study was to evaluate RS174547 in FADS1 gene and RS1260326 in GCKR gene in tow groups Patient and control for identification of related to high triglyceride. The blood samples were collected from 50 patients and 50 controls. The polymerase chain reaction was carried out using genomic DNA. Consequently, PCR production were sequence. The results of this study indicate the relationship between FADS1 and GCKR mutations in patients with high triglycerides. In this study, we investigated the T/C allele in Hyper triglycerides, some of which were pure and some of them gross. In fact, the initial results suggest that this polymorphism has been seen in some control samples, and this is not a symptom of a person s disease, but suggests that the underlying disease.

Keywords:

FADS1 , GCKR , Triglyceride , type 2 diabetes , cardiovascular disease