Evaluation of Genetic variability of 49 informative autosomal SNPs marker in Afghan ethnicity group

Single Nucleotide Polymorphisms (SNPs) in comparison with STRs have a much lower mutation rate and can applied when STR results are not definitive enough in complex relationship tests, also for genotyping severely degraded DNA. The purpose of this study was to analysis the Genetic variability of 49 autosomal SNPs in the Afghan population and we evaluate their helpfulness for applying in forensic casework. A set of 49 autosomal single nucleotide polymorphism (SNP) loci in the SNPforID 52plex databases was analyzed in 55 unrelated individuals from the Afghan population residing in Iran using SNaPshot assay. FIS and FST were calculated. No deviation from Hardy-Weinberg equilibrium (HWE) was observed for any SNP in the Afghan population and no significant pairwise linkage disequilibrium was observed for any SNP pair. FIS and FST were calculated. A multidimensional scale was done from the FST pairwise values to compare the Afghan population with other West and East Asia populations. The Afghan population grouped closely to populations living geographically near to Iran based on pairwise FST distances. Statistical parameters of forensic interest calculated for the Afghan population The mean match probability calculated for 49 SNPs in Afghan. Owing to variability the genetic structure of the Afghan population is sufficiently distant from the Iranian population, it can be potentially used in forensic genetic applications.

Keywords:

Autosomal SNPs , SNPforID , Afghan , Human identification