Study of effect of anticancer) metformin( drug on cell viability assay in T47D breast cancer cell lines on cell culture

Publish Year: 1397
نوع سند: مقاله کنفرانسی
زبان: English
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CIGS15_232

تاریخ نمایه سازی: 13 بهمن 1398

Abstract:

Breast cancer is the most frequently diagnosed cancer and the leading cause of cancer death in females worldwide. Metformin is widely used in the treatment of diabetes mellitus type 2. Epidemiological studies suggest that metformin may be an effective agent in treatment of diabetic patients with breast cancer.Methods:In this study we used the breast cancer T47D cell line. The cell line was incubated in DMEM supplemented with 10% fetal bovine serum under 5% CO2 and 37℃ temperature. Then T47D cell line treatment with three different doses of metformin (5, 10 and 50 mM) in three different times (24, 48 and 72 hours). At least 3 repeated in the same dose of treatment and time done for each group. After of cell harvested, cell proliferation was assessed with methylene blue method. After statistical analysis, pair T test, T test and ANOVA were performed by using of spss software.Results:There results were shown that the significant changes between the control groups and treatment groups. It is concluded that metformin reduce count of the T47D cell line of the breast cancer in a dose-dependent manner.

Authors

Hanzaleh Balakheyli

Golestan Rheumatology research center, Golestan University of Medical Sciences, Gorgan, Iran.

Jahanbakhsh Asadi

Golestan metabolic disorders research center, Golestan University of Medical Sciences, Gorgan, Iran.

Gholamreza Roshandel

Golestan Research Center of Gastroenterology& Hepatology (GRCGH) , Golestan University of Medical Sciences, Gorgan, Iran.