Neda Ebrahimzadeh - Department of Biology, Islamic Azad University of Damghan, Damghan, Iran
Hagar Vaseghi - Optical Restoration Research Group, Laser Research Center, Tehran University of Medical Sciences, Tehran, Iran.
Majid Pournour - Optical Restoration Research Group, Laser Research Center, Tehran University of Medical Sciences, Tehran, Iran.
Gholamreza Esmaili Javed - Optical Restoration Research Group, Laser Research Center, Tehran University of Medical Sciences, Tehran, Iran.
Shahla Mohammad Ganji - National Institute of Genetics and Biotechnology, Tehran, Iran.
Layla Novin - Optical Restoration Research Group, Laser Research Center, Tehran University of Medical Sciences, Tehran, Iran

Introduction: Diabetic severe foot ulcer is a complication in type 2 diabetes. Various factors, such as changes in the level of cytokines (especially IL-1β), can interfere with the development of diabetic severe foot ulcer. Several factors affect the level of IL-1β levels, including the rate of neurotransmitter (and especially dopamine) and its receptors. IL-1β level can be inÏuenced by several factors which is one of them could be related to the rate of some neurotransmitters such as dopamine and their receptors. The aim of this study was to evaluate the changes of IL-1β and dopamine DRD2 receptor in peripheral blood mononuclear cells (PBMCs) in diabetes with severe foot ulcers.Material and methods: Peripheral blood samples were collected from 31 subjects with ulcer, 29 without ulcer and 25 healthy individuals. Total mRNA was extracted from PBMCs and cDNA was synthesized for study of DRD2 and il-1β gene expression variations by real time PCR technique. Consequently, concentration of il-1β was investigated in sera.Results: Significant decrease in gene expression and sera concentration of il-1β in PBMCs was observed in diabetes with severe foot ulcer. There were also a significant reduction in the expression of the DRD2 receptor gene in patients with and without severe foot ulcer.Conclusion: It seems that the reduction of IL-1β expression is related to changes in DRD2 receptor gene expression. Therefore, after supplementary studies, it may be possible to state that reducing the expression of DRD2 as a prognostic factor and as an effective factor in the spread of diabetic severe foot ulcer.

Diabetic severe foot ulcer, Dopamine receptor, Il-1β, Gene expression change